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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白

  • 產(chǎn)品型號(hào):rRtMIP-1β/CCL4
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白與其它公司提供的重組蛋白不同,rRtMIP-1β/CCL4蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白,可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效,將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白(全長(zhǎng)序列)產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹:

重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白

Synonyms Small-inducible Cytokine A4
Species Rat
Accession P50230
GeneID 116637
Source Escherichia coli.
Molecular Weight 重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Quantity 5μg/20μg/1000μg
AA Sequence APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.
Endotoxin Less than 1 EU/μg of rRtMIP-1β/CCL4 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠巨噬細(xì)胞炎癥蛋白-1 beta / CCL4蛋白
Reference 1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.
2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.
3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.
Background Chemokine (C-C motif) ligand 4 encoded by the CCL4 gene, also known as macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL4 contains 69 amino acids and it shares 80% and 86% a.a. sequence identity with human and murine CCL4. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.

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